The NRS-4500 Series is a purpose designed Confocal Raman Microscope with a wide functionality to make setup fast and easy. When changing lasers the selection and alignment of the laser, rejection filter and grating are fully automated.
The laser spot is observed on all models including 1064nm ensuring that the sample being viewed in actually being measured. Sample identification, imaging analysis and identification can all be done in real time, speeding up the process of preparing the sample to seeing the results.
The f200 spectrograph includes up to 4 automatically selected gratings and detection by a high performance CCD or InGaAs array (with options for electron multiplying EMCCDs). The observation system includes a super resolution CMOS camera with Olympus optics and a choice of objectives from x5 to x100. Objective options include NIR and long working distance for working with heating cooling/sample stages.
The standard Class 1 safety cabinet.
An automatic XYZ stage is used for confocal imaging with X/Y spatial resolution to 1 µm (Z=1.5 µm). Spectra Manager™ Suite with the Imaging Analysis application is used to collect a three dimensional image of the sample which can be rotated and viewed from any angle. The image map is created using peak data corresponding to key functional groups and analysis of multiple peaks can be combined to create powerful data images.
The NRS-4500 Raman Microscope offers several standard configurations including the typical 633nm single laser or 532/785nm laser combination with matching notch or edge filters, with an option for a third laser which can be mounted internally or externally. Observation of the laser spot ensures perfect alignment of the target sample position and provides an indication of surface roughness. All laser wavelengths are selected in the software and once selected, the optical system (including the laser) is automatically aligned for optimal throughput and resolution. Four software selectable gratings control the spectral range and resolution from 8000 to 100 cm-1 (8000 to 50 cm-1 as an option). The grating direct drive system includes a rotary encoder to ensure excellent wavelength reproducibility ±0.2cm-1.
JASCO has developed new and novel (patented) mechanisms to deal with sample fluorescence. As with other Raman systems, we can utilize laser wavelengths of 785 nm and up to 1064 nm, but we have recently incorporated a 457 nm laser that offers significantly higher Raman signal, improved spatial resolution and much lower fluorescence for many different sample types. Selecting a different excitation laser wavelength is only one of the ways the NRS-4500 minimizes fluorescence interference. The Fluorescence Rejection algorithm (patented) included in the Spectra Manager™ can effectively remove or minimize fluorescence regardless of the laser wavelength used either at the time of measurement or during post-processing.
The ‘UserAssist’ software aids the user in setting up the NRS-4500 for sample measurement; a simple sequence guide takes you through setup and optimization of measurement parameters with helpful advice and tips, such as a warning if you have the laser intensity set too high. When each of the parameters has been set, the NRS-4500 automatically selects the laser and matching notch filter, the grating for the appropriate resolution, focuses on the sample and then the spectral measurement is performed.
The new ‘Sample Search’ function is used with the automated XYZ stage. A new algorithm developed by JASCO (patent pending) analyzes the microscopic image and automatically selects measurement position(s) based on size, contrast and/or color of the target material. The user simply clicks the measurement button to execute spectral measurements of the desired sample positions.
To provide faster Raman microscope image processing, the Imaging Analysis software includes a ‘Registry’ of possible functional groups or other relevant compound information based on peak height or area calculations. After peak height or area calculation is developed, it can be saved to the Registry for future analysis use. The registry includes the peak calculation information and a ‘label’ describing the relevant vibrational motion. Image maps can be developed from mapping data simply by clicking on the desired, registered calculation
The NRS-5000 is a research grade confocal Raman microscope with f300 spectrograph that combines the flexibility of multiple detectors and multiple laser sources to quickly change between measurement modes for fast data acquisition to reduce experimental time.
A rigid honeycomb optical base is the foundation of the instrument ensuring alignment and stability. The laser(s), microscope, software-switched optics and a unique aberration-corrected polychromator with CCD or InGaAs detectors provide an integrated compact package that is . Operator safety (Class 1 laser safety) is maintained by a fully enclosed automated sample chamber door. This provides a 120 degree opening for the user to freely access the sample stage and objectives revolver when setting up the experiment.
A high resolution color CCD viewing system with synchronized image capture and spectrum measurement enables accurate sample identification and positioning. All data is recorded in the Spectra Manager™ imaging application. An automated 4 position grating selector, 2 internally mounted detectors and up to 8 lasers ranging from the UV through the NIR. The NRS-5000 is software controlled for maximum flexibility so that once the sample is loaded onto the sampling stage the user has complete control under conditions of class 1 laser safety.
The NRS-7500 is a research grade confocal Raman microscope with f500 spectrograph that combines the flexibility of multiple detectors and multiple laser sources to quickly change between measurement modes for fast data acquisition to reduce experimental time.
NRS-7500 Raman instrument includes a color CCD sample viewing system with image capture capability. For additional flexibility, a trinocular microscope attachment can be added for direct binocular viewing.
For application expansion, an automated multi-grating turret, 2 internally mounted detectors and a maximum of 8 lasers ranging from the UV through the NIR are capable of integration with the instrument system. All optical components are PC controlled for maximum flexibility with minimum user interaction.
A number of unique technologies have been developed to improve measurement efficiency